| Summary: | 碩士 === 國防醫學院 === 生理學研究所 === 90 === The predominant hemodynamic changes in sepsis and endotoxemia are systemic hypotension and tracheal contraction. Thus we explored the differences among aortic smooth muscle cells of aorta, pulmonary artery, and trachea. In this study, the nitrite accumulation and calcium mobilization (induced by 30 microM ATP), detected by a calcium-sensitive fluorescent dye (fura-2), among cultured tracheal, pulmonary artery, and aortic smooth muscle were evaluated during LPS exposure. And the result indicated that LPS increased nitrite accumulations in supernatant in a time-dependent manner. However, after exposure with LPS (100 microgram/ml) for the nitrite accumulation in TSMCs (12.03 ±0.70 microM) was significantly lower than in PASMCs and ASMCs (30.02 ±1.68 and 30.44 ±2.38 mM). The resting [Ca2+]i in TSMCs, PASMCs and ASMCs were 0.589 ±0.009, 0.558 ±0.006 and 0.585 ±0.008 (F/Fo) respectively. And after LPS treatment the resting [Ca2+]i were 0.572 ±0.009, 0.573 ±0.007 and 0.582 ±0.007. The amplitude of calcium mobilization (peak [Ca2+]i minus resting [Ca2+]i) after LPS treatment was significantly increased from 0.47 ± 0.041 to 0.697 ±0.033, 0.486 ±0.03 to 0.646 ±0.02 and 0.527 ±0.027 to 0.618 ±0.028 (ΔF/Fo) respectively. To conclude, the modulation of intracellular calcium might be influenced by nitric oxide with different degree in vascular and tracheal smooth muscle cells.
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