Characterization of an Iron-responsive Promoter in the Protozoan Pathogen Trichomonas vaginalis

• Main Authors: Tsai Chu-Dang, 蔡住當
• Other Authors: Tai Jung-Hsiang
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/05185114695668240340

博士 === 國防醫學院 === 生命科學研究所 === 90 === 英文摘要 Iron is a key modulator of gene expression in Trichomonas vaginalis. In this study, iron induced gene regulation of T. vaginalis adhesin protein（AP65-1）was investigated. A transient DNA transfection system was established and for the analysis of ap65-1 promoter activity. The region -109/-56 was found to be critical for iron-induced transcription and -121/-102、-52/-39 and initiator element were found to be important for basal transcription. Nuclear proteins binding sites in these regions were identified by electrophoretic mobility shift assay, was shown to be -95TAACGATAT-87 and T-rich sequence in the distal promoter region. According to these results, DNA transient transfection system is usfeul to further study basal and iron-induced transcriptional regulation in T. vaginalis.