| Summary: | 碩士 === 國立東華大學 === 化學系 === 90 === A Polyaniline/Nafion（Pan/Nafion）carbon paste electrode was prepared by mixing the polyaniline emeraldine salt powder with nafion and graphite to construct the urea & ammonia sensor. the urease was immobilized on the controlled porous glass（CPG）as the enzyme reactor and amounted before the Pan/Nafion electrode in flow injection analysis (FIA) system to analyze the urea in blood samples .The ratio of nafion, polyaniline and graphite for the construction of the electrode and the optimal conditions were studied. The detection limit of this sensor for urea was 5 �嵱 and the linearity from 5 �嵱 to 7.5 mM was obtained in FIA. The reproducibility and stability were acceptable (RSD, 6.3 %, n=43) and the response time ca 72s. The real samples from blood dialysis patients were analyzed and compared with the urea test kit method.
A nicotinamide adenine dinucleotide (NAD+) dependend glutamate dehydrogenase (GlDH) was studied for glutamic acid analysis. The GlDH ， NAD+， Pan， Nafion was mixed to preparaed the GlDH/NAD+/Pan/Nafion paste electrode. The ratio of GlDH and NAD+ for the Pan/Nafion electrode and the optimal conditions were studied. The detection limit of this sensor for glutamic acid was 500 �嵱 and the linearity from 500 �嵱 to 5 mM was obtained in FIA. The reproducibility was acceptable (RSD, 4.25 %, n=40).The real samples from food additive were studied.
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