Attenuated total reflection biosensors

• Main Authors: Chien-Hung Tsai, 蔡建宏
• Other Authors: S.-J. Chen
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/84913927090396855420

碩士 === 國立中央大學 === 機械工程研究所 === 90 === The biosensors based on surface plasmon resonance (SPR) has been often used as tools for directly detecting the kinetic interaction of unlabelled biological molecules at surface in real time. The SPR biosensors have many advantages over others, such as the high sensitivity, label free, kinetic study, and high-throughput screening etc.. The potential of this technology is gradually being noticed in the field of biological diagnostic. The resolution of detector which is made by the method of attenuated total reflection is often limited by the goniometer. The topic in this paper is to efficiently use a novel resolution of the goniometer to improve sensitivity and resolution. Use design of optical system and multi-layer sensors, we can measure the reflected light, both TM and TE modes, simultaneously, use less detector time and frequency get more information and we can modify SPR angle. When the dielectric constant and thickness of unknown medium problem are solved, we can reduce the tolerance under 5% by optimal linear data analysis with the data solved by Lorentzian type relation and Fresnel equations. The advantage of this biosensor is that we can get kinetic study by using this system, and analyze molecular concentration and thickness. Therefore, the traditional fluorescence can not achieve this requirement.