Application of Surfactant-Containing Refrigerant R134a to Extract Hydrophilic Compounds in Countercurrent Chromatography

• Main Authors: Chun-Ju Han, 韓淳如
• Other Authors: Tiing Yu
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/56786421236854275716

碩士 === 國立交通大學 === 應用化學系 === 90 === We try to use surfactant-containing refrigerant R134a (1,1,1,2-tetrafluoro-ethane) to extract hydrophilic matters in High-Speed Countercurrent Chromatography (HSCCC) in this study. Because R134a can separate with extracts without any concentration after degassing under ambient condition , it is here in use to replace organic solvent in reversed micelle extraction. Complete mixture of R134a and aqueous solution in HSCCC enhaces extraction efficiency. After pressure decreasing at the end of column in HSCCC is overcome, we extract methyl orange from aqueous solution successfully with liquid R134a containing cationic surfactant, trioctylmethylammonium chloride(TOMAC), and concentrate it in small volume of collecting liquid after releasing R134a at the end of column. When using R134a under prsssure,17bar, containing TOMAC which volume concentration is 2% to extract methyl orange from aqueous solution, recovery is estimated to be 80% .We cannot extract proteins in this apparatus due to the deficiency of prssure endurance of column in HSCCC.