Statistical Analysis of the Gene Expression for Non-synchronized Cell Cycles of Human Glioma Cells after Gamma Irradiation by cDNA Microarray

• Main Author: 戴郁佳
• Other Authors: 盧鴻興
• Format: Others
• Language: en_US
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/75330224901137425536

碩士 === 國立交通大學 === 統計所 === 90 === Microarray is a high throughput technique. We can observe a large number of gene expressions by this new technique in a short time. In order to understand the gene expression profiles in different phases of cell cycles of human glioma cells after gamma irradiation, microarray experiments are performed in Professor Ngo’s laboratory at National Yang-Ming University (Ngo, Chan, Chang, 2001). The experiments are non-synchronized to imitate the in vivo expression pattern. Furthermore, these will provide fast and economic approaches to screen the gene functions. However, these data bring us challenges in analysis. This study is an attempt to separate the effect of non-synchronization from the genuine cell cycle function by statistical methods for microarray data with cell proportions measured by flow cytometry.