| Summary: | 碩士 === 國立成功大學 === 製造工程研究所碩博士班 === 90 === The normal human growth includes the qualitative and quantitative differences, the former means to the cell differentiation, and the latter means to the cell proliferation. From the fertilization, the germ cells grow to a new individual by cell proliferation and differentiation. Gene expression means to the result of a series of interactions between proteins and codes on DNA of the chromosome. Cell differentiation and proliferation are a series of process of gene expression regulation.
As time goes on, genes increasingly express various cell types in order in cell proliferation and differentiation. Since genes on DNA determine the results of cell differentiation and proliferation. Genes on DNA determine to turn on or off by transcription factors. However, the conformations of these transcription factors attached to DNA involve in chemical interactions between DNA and transcription factors, because any biochemical reactions in vivo involve in energy change. Of course, the chemical bond continuously form and destroy between double helices of DNA and transcription factors.
Owing to the researches in proteins’ functions are increasingly developing in the world, the related researches are rare to analyze the entire differentiation and proliferation. Therefore, it is necessary to analyze the entire 3-D conformations of proteins so that we can recognize clearly the important factors and general principles. By the analysis of energy, to calculate the energy difference from beginning maybe offer to another opinion of controlling cell proliferation and differentiation. In this thesis, we preliminary focus on the 3-D conformation of proteins participating in cell differentiation and proliferation in level of the molecular, and further we will search for bond-selective, region-selective or mode-selective means to modify and manipulate biochemical reactivity in engineering. We further use the 3-D software of molecular level and minimum static energy to analyze the three-dimension conformation, then, find out the sufficient and necessary conditions to explain the process of differentiation and proliferation.
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