| Summary: | 碩士 === 國立成功大學 === 機械工程學系碩博士班 === 90 === DNA is the basic unit which contains gene in cells and controls the human body’s health and gene change. Therefore, one can detect diseases and identify gene by effectively screening DNA base. With the new advent in new medicine and identification of the blood relationship, faster and simpler DNA screening methods are urgently needed. There are several problems in traditional dyeing and fluorescence in DNA screening method that include (1) the precision of fabrication is difficult to control, (2) the screening process is too complex, and (3) cost is too high. These problems limit new developments in gene engineering. Therefore, it is very important to find a new DNA screening method that is simple to operate and cost effective. Some experts combine nanoparticles with probe DNA and screen DNA by different colors between the dispersal and aggregation of nanoparticles. This new approach has many advantages such as fast structure identification, clear colorimetry, fine selectivity, and less experiment equipment. Since the aggregation of the self-assembly of DNA nanoparticles is too complex, it is difficult to describe the process. Even now there is no suitable theories and experiments to prove its practicality. In order to increase the accuracy of the DNA screening system, the characterization of optical properties of the self-assembly of nanoparticles has become more and more important.
To solve the aforementioned problem, this paper will explores and studies the self-assembly of nanoparticles and the associated optical properties that can be applied to detect human’s diseases. First, the energy approximation to the self-assembled of DNA nanoparticles is established and the employment of this approximation to analyze the physical rules of DNA self-assembly nanoparticles in micro-viewpoint is provided. Secondly, the nanopartcles polarization technique is used to establish theoretic optical structures and to forecast the optical spectrum of DNA nanoparticles. The results of self-assembly energy approximation and the complete diagnostic rules for DNA structural characterization are presented. The methods that were used to differentiate DNA complementary and measure DNA base number for diagnosing the diseases about DNA base mutation are introduced. Finally, the suggestions to improve the sensitivity of self-assembly of optical nanoparticles in DNA screening system are proposed and implemented.
Good agreements between the computed solutions and existing data obtained from the literature indicate that the proposed theory and the modeling procedure is theoretically sound and practically applicable for computing the optical spectrum of self-assembled DNA nanoparticles screening system. Based on the results obtained from examples presented in the paper, one can realize that the proposed methods can differentiate DNA complementary and also measure DNA base number to diagnose the disease about DNA base mutation. Furthermore, the computed results are accurate enough for diagnosing the disease. The proposed error adjustment scheme for improving DNA screening process can actually promote the sensitivity for identification of the structures of self-assembled optical nanoparticles in DNA screening system.
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