Effects of LDL on Leukocyte- or Platelet- Endothelium Interaction

• Main Authors: Wen-Gia Fan, 范文佳
• Other Authors: Hsiun-ing Chen
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/73a3w9

碩士 === 國立成功大學 === 生理學研究所 === 90 === Atherosclerosis is a chronic inflammatory disease. Previous studies have indicated that abnormally elevated concentrations of plasma LDL will result in an increase in rolling/adhesion of leukocyte and the expression of the relevant adhesion molecules. The existing evidence suggests that the treatment of native LDL (nLDL) in cultured human vascular endothelial cells will induce calcium transient and trigger vascular cell adhesion molecule-1 (VCAM-1) and E-selectin expression. However, the role of nLDL in the interaction of leukocytes/platelets and endothelial cells is largely unknown. To investigate whether the endothelium- leukocyte or platelet interactions could be altered by nLDL, cultured human umbilical vein endothelial cells (HUVECs) were treated with nLDL. Leukocyte and platelet adhesion to HUVECs was subsequently measured by using a tapered flow chamber that has a linear variation of shear stress across its flow channel. To verify which adhesion molecules are involved, the immunostaining were executed. Our data showed that 1) the pretreatment of high dose of nLDL (1.4mg/ml) would change cell morphology of HUVECs; 2) leukocytes, especially monocyte, but not neutrophil , adhered more readily to nLDL-treated HUVECs than to the control group at all shear stress levels tested; 3) the adhesiveness of monocyte on endothelium was higher than that of neutrophil; 4) the expression of P-selectin and VCAM-1 in HUVECs increased after nLDL pretreatment. We conclude that nLDL pretreatment enhances leukocytes adhesiveness, especially monocyte, and that P-selectin and VCAM-1 may be involved.