Mechanism-Based Inactivation of Metapyrocatechase – Synthesis of 3-Aminomethylcatechol and Enyzmatic Reaction Mechanism
碩士 === 國立成功大學 === 化學系碩博士班 === 90 === The target substrate 3-aminomethylcatechol was prepared from imine derivative, which was in turn synthesized from the condensation of 2,3-dihydroxybenzaldehyde with ammonia. 3-aminomethylcatechol was proved to be a competitive inhibitor of metapyrocatechase....
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2002
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| Online Access: | http://ndltd.ncl.edu.tw/handle/95jppy |
| Summary: | 碩士 === 國立成功大學 === 化學系碩博士班 === 90 === The target substrate 3-aminomethylcatechol was prepared from imine derivative, which was in turn synthesized from the condensation of 2,3-dihydroxybenzaldehyde with ammonia.
3-aminomethylcatechol was proved to be a competitive inhibitor of metapyrocatechase. The enzyme mediated oxidation product was showed an intense absorption at 395 nm with a KM of 21.42 mM.
While incubated the metapyrocatechase with 3-aminomethylcatechol, the result revealed a a pseudo first-order time-dependent loss of enzyme activity, this result indicated that 3-aminomethylcatechol was a mechanism-based inactivatorof metapyrocatechase (KI = 39.85 mM，kinact = 1.29 min-1). A plausible inactivation mechanism was proposed.
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