Simulation and Analysis of heating mechanisms of the Polymerase Chain Reaction Chip

• Main Authors: Chung-Che Yang, 楊宗哲
• Other Authors: Yu-Cheng Lin
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/tyxhvm

碩士 === 國立成功大學 === 工程科學系碩博士班 === 90 === This study used the finite element method (FEM) to simulate and analyze the transient temperature distributions in the micro polymerase chain reaction (PCR) chip. The contact and non-contact heating mechanisms were considered and the temperature uniformity, distribution, heating and cooling rates were investigated herein. When the contact heating mechanism was considered, the distribution temperature of the reaction chamber is uniform. The numerical results were also confirmed by one-dimensional theoretic analysis. The improved thermal cycle of the rapid micro-PCR system were verified using serum sample form patients with chronic hepatitis C. The simulated results were used to improve the thermal cycles time of a rapid mPCR system. As the non-contact heating mechanism was considered, the size of the reaction chamber and the wavelength of the optical source influenced the heating/cooling rate under the same amount of the incidence energy. Four different wavelengths of the optical sources and three different sizes of the reaction chamber were employed in the present study. For several wavelengths of the optical sources, the energy deposited only in the silicon layer. For the other wavelength, energy deposited in both silicon and reaction chamber. The temperature distributions under contact and non-contact heating mechanisms have been simulated and discussed in the present work.