純菌株對於多環芳香探清化合物-奈、菲分解能力之研究

• Main Author: 李孟哲
• Other Authors: 盧至人
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/54844239649029258468

碩士 === 國立中興大學 === 環境工程學系 === 90 === ABSTRACT The objective of this study was to assess the biodegradation of polycyclic aromatic hydrocarbons (PAHs) by the acclimated bacteria. This study focused on the rate of biodegradation、the growth rate、the specific enzyme activity、the oxygen uptake rate and environmental factors for PAHs biodegradation. Three acclimated PAHs-degrading strains，Comamonas testosteroni，Rhodococcus rubber and Serratia marcescenswere，were isolated from a chemostat，which was continuously fed with naphthalene and phenanthrene as the substrates. In the specific enzyme activity tests，the experimental results indicated that the value of Km were 0.26、0.19 and 0.24 μmole；the value of Vmax were 0.00047、0.00084 and 0.00056 μmole/min for these three species，respectively. In the biodegradation rate and growth rate tests，experimental results indicated that the value of Y were 0.77、0.63 and 0.87；the value of k were 0.35、0.29 and 0.31 hr-1 and the value of k/Y were 0.45，0.47 and 0.35 hr-1. for these three species respectively. The ratios of the theoretical oxygen demand to the experimental oxygen demand obtained from a respirometer with soluble NAP as the substrates were 0.77、0.66、and 0.91 for these three species，respectively. However the ratios were 0.29、0.97 and 0.87 for these three species，respectively when coystal NAP was the substrate.When phenanthrene was used as the substrates，the ratios were 0.01、0.08 and 0.02 for these three species，respectively. For environmental factors stady，the experimental results indicated that these three acclimated degrader grew batter in the temperature at 34℃ and pH at 6.4，especially using ammonium as the nitrogen sources. Keyword : naphthalene，bioremediation，specific enzyme activity