| Summary: | 碩士 === 國立中興大學 === 農藝學系 === 90 === Summary
The immature embryos of interspecific hybrids between cultivar TN11 and six wild type（A. cardenasii, A. rigonii, A. chacoense, A. corrotina, A. spegazzinii and A. monticola） were cultured on MS＋4 mg/l TDZ to induce multiple shoot and bud primordia. The collected bud and bud primordia was them used to investigate the effects of TDZ on shoots induction and proliferation, and to establish plant regeneration technique. The results were summarized as follows:
1. Buds of six interspecific hybrids cultured on MS medium containing different TDZ concentrations to induce multiple shoot. The induced percentage of shoot were significantly different among TDZ levels and crosses, but the averaged shoots were non-significant different. The mean numbers of bud primordia cultured on MS media supplemented with TDZ were significantly higher than on MS medium. Bud primordia cultured on MS media containing TDZ could induce shoots, but the efficiency was lower than buds as explants.
2. In all crosses, the induced percentage of shoot induced from shoot through three times subculture on MS＋2 mg/l TDZ medium could maintain above 50%, and the averaged shoot was stabilized.
3. TDZ induced the small and thickened multiple shoots, but MS medium without TDZ could increase the induced percentage of shoot and obtained normal shoots. Shoots subcultured with the MS medium without TDZ have the higher rooting percentage compare to MS medium containing TDZ, and root system was more healthy.
4. If the shoots of six interspecific hybrids transferred to MS media containing 50 mg/l ascorbic acid and three levels of NAA, the rooting percentage increased with NAA concentration. The MS medium containing 0.5 mg/l NAA produced the highest percentage of rooting and have more healthy root system. The percentage of rooting of all interspcific crosses were reduced with the subculture generation increased.
5. The recovery percentages of the rooted plantlets were ranged from 32.16% to 54.66% in six interspecific crosses. These plants were demonstrated as interspecific hybrids by chromosome number and morphological characteristics.
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