Apoptosis in Human Hepatoma Cell Line Induced by Luteolin

• Main Authors: Chien-Hsuan Chen, 陳鍵萱
• Other Authors: Chun-Ching Lin
• Format: Others
• Language: en_US
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/42595086986851299512

碩士 === 高雄醫學大學 === 天然藥物研究所 === 90 === In order to develop therapeutical drugs for liver cancer, we evaluated the cytotoxic effect of luteolin, 2-(3,4-Dihydro xyphenyl)-5,7-dihydroxy-4H-1- benzopyran-4-one, on human hepatoma cell lines. Five different human hepatoma cell lines, namely HepG2, SK-HEP-1, PLC/PRF/5, Hep 3B and HA22T/VGH were used as target cells. The colorimetric assay that involved reduction of XTT tetrazolium to soluble orange formazan was used to evaluate the cytotoxic activity of luteolin. The results indicated that luteolin exhibited potent anti-hepatoma activity, and also found that luteolin expressed different levels of cytotoxicity on five human hepatoma cell lines. According to the results, luteolin displayed predominant cytotoxicity against HBV-DNA(+) hepatoma cell lines of PLC/PRF/5 and Hep 3B with IC50 values of 8.40, 15.04μg/ml, respectively. Besides, this study showed that luteolin-treated PLC/PRF/5 cells exhibited typical changes of apoptosis. Flow cytometric analysis exhibited a prominent apoptotic marker (sub-G1 peak) after luteolin treatment. After gel electrophoresis, the fragmented DNA showed a characteristic ladder pattern. Cell cycle analysis revealed that luteolin induced PLC/PRF/5 cell cycle arrested at G0/G1 phase.