Effect of Antrodia camphorata on Plasma Glucose and Antioxidation in STZ-Induced Hyperglycemic Rats

• Main Authors: Kuei-Rong Yen, 嚴貴榮
• Other Authors: Chingmin E. Tsai
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/88566039119117435098

碩士 === 輔仁大學 === 食品營養學系 === 90 === The aim of this study was to investigate the physiological effect of A. camphorata on blood glucose, lipids and antioxidation in hyperglycemic rats. Forty eight hyperglycemic rats were induced by injections of streptozotocin (STZ)（25 mg/kg body weight, every three or four days until blood glucose was induced to 200∼300 mg/mL）and fed high sucrose diet to maintain blood glucose levels, then were divided into six groups treated with control, cultural medium (medium-An5), fermentative filtrate (filtrate-An5) of A. camphorata An5 or cultural medium (medium-GK101), fermentative filtrate (filtrate-GK101), mycelia (mycelia-GK101) of A. camphorata GK101 diet. Animals were sacrificed, and their blood and liver were analyzed after feeding for four weeks. Results showed that feeding of either mycelia-GK101 or filtrate-An5 diet could significantly decrease non-fasting plasma glucose (p<0.05). It also demonstrated that feeding of filtrate-An5 or filtrate-GK101 diet could significantly decrease fructosamine (p<0.05). In the response of oral glucose tolerance test (OGTT), feeding of mycelia-GK101 or filtrate-An5 diet could significantly decrease plasma glucose and insulin (p<0.05). According to the serum lipid analysis, less triacylglycerol and total cholesterol were noticed in rats treated with filtrate-An5 than those with medium-An5 (p<0.05). No significant differences were found in serum LDL-cholesterol, HDL-cholesterol and free fatty acids levels among all groups (p>0.05). Moreover, the hepatic TBA reactive substances (TBARS) in the group of filtrate-GK101 were lower significantly than the one of mycelia-GK101 (p<0.05). No significant difference, however, on the lag time of Cu2+-induced LDL oxidation, 50% hemolysis of red blood cells and total antioxidant status were detected among all groups (p>0.05). The observations can be concluded that both fermentative filtrate and mycelia of A. camphorata could reduce the blood glucose. The filtrates could decrease the abnormal concentrations of serum lipids and ameliorate the oxidative stress in liver. Subsequently, A. camphorata may be considered a nutraceutical to improve diabetes. The study also revealed that various cultured A. camphorata products might have various levels of physiological effects.