Simulantaneous Determintation of Fives Preservatives and Two Artificial Sweeteners by HPLC Method

• Main Authors: Tung-Huan Lin, 林東煥
• Other Authors: Ceu-SHing SHeu
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/04171272039583667020

碩士 === 朝陽科技大學 === 應用化學系碩士班 === 90 === This program probes into the method of simultaneously examining five preservatives:p-Hydroxy Benzoic Acid, Salicylic Acid, Benzoic Acid, Sorbic Acid, and Dehydrate Acetic Acid, and two artificial sweeteners: Saccharin and Dulcin by HPLC. The solid samples were shacked and extracted by water, and then filtered. The liquid samples were diluted with water and then filtered. After these procedures, they were injected into HPLC. The consistencies of p-Hydroxy Benzoic Acid, Salicylic Acid, Benzoic Acid, Sorbic Acid, Dehydrate Acetic Acid, Saccharin and Dulcin will be presented in twenty minutes by using a Intersil-ODS2-6mmx15mm 5um column, and the buffer solution of Methanol : Acetonitrile : 5mM citric Acid buffer (15:15:70) as mobile phase; under the condition of 1ml/min flow velocity, and 225nm wavelength for detection. The linear correlation factor reached r2=0.9994~0.9998. LOD is 1~2ng, under the condition of signal to noise ratio (S/N)=3. When S/N=10，LOQ is 1~2 mg/kg. The average recovery of adding appropriate standard solution into solid and liquid samples reaches 97.57%. In total sixteen products tested by this method, there were eleven solid samples containing preservatives or artificial sweeteners, which were over the limit, and three liquid samples containing preservatives, but the additive quantities were within the allowable range, and the others contained no preservatives or artificial sweeteners.