Effect of a-lipoic acid on pheomelanogenesis of cultured B16-F1 melanoma cells.

• Main Authors: Shan-Yu Ho, 何賢毓
• Other Authors: Han-Hsin Chang
• Format: Others
• Language: zh-TW
• Published: 2002
• Online Access: http://ndltd.ncl.edu.tw/handle/53279465626674585191

碩士 === 中山醫學大學 === 營養科學研究所 === 90 === Melanin is a complex composed of pheomelanin and eumelanin in nature, and its ratio of content is mainly regulated by glutathione. Pheomelanin and eumelanin ratio is an important risk factor of skin cancer. It has been confirmed that thiol antioxidant such as a - lipoic acid could increase glutathione content in cells, and therefore our study was to evaluate [1] the growth and intra/extracellular total melanin content of B16-F1 melanoma cells when treated with different concentrations of a - lipoic acid at 24, 48, and 72 hours intervals, [2 ] the intra/extracellular pheomelanin, eumelanin level, and pheomelanin / eumelanin ratio of B16-F1 melanoma cells 72 hours after being treated with a - lipoic acid, and [3] the intra/extracellular total melanin, pheomelanin, eumelanin, and pheomelanin / eumelanin ratio of B16-F1 melanoma cells 72 hours following the treatment of same concentration of a - lipoic acid, vitamin C phosphate magnesium, and vitamin E succinate. Our results revealed that [1] a - lipoic acid increased the growth of B16-F1 melanoma cells, and decreased intra/extracellular total melanin content, [2] a - lipoic acid increased intracellular pheomelanin content, intra/extracellular pheomelanin/eumelanin ratio, and decreased intra/extracellular eumelanin, intracellular pheomelanin content, and [3] vitamin C phosphate magnesium or vitamin E succinate both increased intracellular total melanin and eumelanin content, and vitamin E succinate considerably increased pheomelanin / eumelanin ratio up to 2.9 fold. Based on these results, we suggest that while use a - lipoic acid and/or vitamin E succinate as inhibitor(s) of total melanin, attention should be simultaneously addressed on the increasing risk of skin cancer.