| Summary: | 碩士 === 中國醫藥學院 === 醫學研究所 === 90 === Activator protein 1(AP-1), a nuclear transcription factor, plays important roles in cell proliferation, differentiation, and transformation. Previous study indicated that blocking of AP-1 activity is able to inhibit the cellular transformation. We therefore develop the anti-human hepatocellular compounds using AP-1 on the target in this study. Effects of 98 herbs on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced AP-1 activity in Chang/AP-1 and HepG2/AP-1 recombinant cells were investigated. Among the herbs, 10 herbs, which showed inhibition abilities on AP-1 activity, were selected. These herbs include Ephedra sinica, Evodia rutaecarpa, Magnolia officinallis, Rheum officinale, Arcea catechu, Stephanica tetrandra, Anemarrhena asphodeloids, Gledititsia sinensis, Sophora flavescens, and Glycyrrhiza uralensis. These herbs were further analyzed for their anti-AP-1 activities and anti-hepatocellular transformation activities by luciferase assay and anchorage-independent transformation assay. Results showed that Evodia rutaecarpa exhibited the significant inhibition ability on AP-1 activity and hepatocellular transformation in a dose-dependent manner. Moreover, the methanol, acid alcoholic, and alcoholic extracts of E. rutaecarpa were responsible for their anti-hepatocellular transformation abilities.
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