| Summary: | 碩士 === 中國醫藥學院 === 中國藥學研究所 === 90 === Rutin is a flavonoid glycoside with many biological activities and is abundant in Huaimi (Sophora japonica L). The oral pharmacokinetics of rutin, Huaimi decoction and commercial extract were studied and compared in rats. The result showed that the absorption of Huaimi decoction or commercial extract was better than that of rutin, and the absorption of commercial extract was superior to decoction.
Cyclosporine is a commonly used immunosuppressive agent with narrow therapeutic range. Its metabolism and absorption is related to CYP3A and P-glycoprotein. It was reported that quercetin affected the activity of CYP3A and P-glycoprotein. In this study, the rats were given cyclosporine alone and coadministration with quercetin, Huaimi decoction, respectively. The blood cyclosporine concentration was determined by fluorescence polarization immunoassay. The result showed that the mean area under blood concentration-time curve of cyclosporine was decreased by 43.3 % after coadministration of quercetin, and by 63.3 % after coadministration with Huaimi decoction.
NO, TNF-α and IL-12 secreted by phagocytes are important cytokine signals in inflammation. The effect of quercetin conjugates on the production of NO, TNF-α and IL-12 by activated macrophages was investigated in vitro. Quercetin glucuronides/sulfates was prepared by using rabbit. The results indicated that quercetin conjugates inhibit the production of NO, TNF-α and IL-12. Moreover, the effect of Huaimi decoctionon on the phagocytic activity of macrophage in the blood was studied by its daily administration to mice for up to 3 months. The result showed that the phagocytic activity was decreased. Both in vivo and in vitro studies implied that Huaimi might affect the immune response through regulating the function of phagocytes.
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