The Effects of Radiation and TNP-470 on KHT Sarcoma of C3H Mice

• Main Authors: LienHui Li, 李蘭蕙
• Other Authors: Yueh Hsing Ou
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/91022667079480651841

碩士 === 國立陽明大學 === 醫學生物技術研究所 === 89 === Angiogenesis has been demonstrated essential for tumor growth and metastases. Inhibition of angiogenesis, so called “anti-angiogenesis therapy” has emerged as a promising strategy for treating cancer since last decade. In these studies, we have examined the effects of Co 60 radiation and an antiangiogenic agent, TNP-470, on KHT sarcoma injected in the hind limb of C3H mice. Our morphologic evidences demonstrated that the death of tumor after radiation went through both dying mechanisms：necrosis and apoptosis. The degrees of necrosis and apoptosis caused by radiation were both dose- and time-dependent. Irradiation and TNP-470 decreased the vessel density of tumor in C3H mice. On day 35 after tumor cells injection, the average vessel number of one 200 X field vision in control mice was 38.4 ± 1.6. Whereas, 15 Gy radiation, TNP-470 alone and 15 Gy together with TNP-470 was significantly lower as 10.8 ± 0.8, 13.0 ± 4.0 and 8.2 ± 1.2 respectively (n = 2) (p < 0.05). Combined therapy using irradiation and TNP-470 prolong the lifetime of tumor-bearing C3H mice from 34.8 ± 6.1 days to 63.3 ± 3.8 days and 75.0 ± 11.7days (n = 4) (p < 0.05). Irradiation and TNP-470 also decreased the extent of lung metastases. On day 35 after tumor cells injection, the number of lung colony in control mice was 32.0 ± 16.9. Whereas, 15 Gy radiation, TNP-470 alone and 15 Gy together with TNP-470 was significantly lower as 12.3 ± 8.4, 9.8 ± 4.7 and 1.8 ± 1.7 respectively (n = 4) (p < 0.05). Taken together, radiation and TNP-470 decreased the vessel density of tumor, lung metastasis and prolong the survival in tumor-bearing mice.