Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16
碩士 === 大同大學 === 生物工程研究所 === 89 === Abstract In the process of organic wastewater treatment, the denitrification using cells of Pseudomonas fluorescence TIT16 was investigated. Isopropanol served as electron donor and carbon source for denitrification. Three kinds...
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ndltd-TW-089TTU001060072015-10-13T12:14:42Z http://ndltd.ncl.edu.tw/handle/64316608452642299812 Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 利用PseudomonasfluorescenceTIT16進行有機廢水脫氮之研究 Hsieh Pei-Hua 謝佩華 碩士 大同大學 生物工程研究所 89 Abstract In the process of organic wastewater treatment, the denitrification using cells of Pseudomonas fluorescence TIT16 was investigated. Isopropanol served as electron donor and carbon source for denitrification. Three kinds of models, including a stirred tank batch reactor, a MBR system and an up-flow fluidized bed reactor, were provided for the studies of the optimum denitrification. For stirred tank batch reaction, the optimum pH was 7.5. After a 12-hour denitrification at pH 7.5, nitrate-N dropped from 140 to 10 ppm. Whereas nitrite was not detected and bacteria sustained life throughout the denitrification process. At pH 6.5, under a slightly acidic condition, the decrease of nitrate-N occurred slowly. The decrease of nitrate-N began at hour 8 and 50 ppm nitrate-N was left behind at hour 12. Meanwhile, the bacteria cells have decayed. At pH 8.5, under a slightly alkaline condition, nitrate-N held still and the bacteria died after a 24-hour denitrification was carried out. By MBR system at a bacteria dose of 0.54 g l-1 cell dry weight, the maximum dilution rate was 8 d-1 for the feeding of wastewater containing 80 ppm nitrate-N. The nitrate-N of the effluent was as low as 10 ppm and the bacteria cells kept alive. With the feeding of wastewater containing 80 ppm nitrate-N, when the dilution rate was shifted to 10 d-1, 40 ppm nitrate-N in the effluent was detected and the bacteria was dying according to the decrease of OD600 value from1.4 to 0.2. In the case of wastewater containing 60 ppm nitrate-N and at a dilution rate of 10 d-1, the effluent contained a nitrate-N below 10 ppm and the OD600 value held at 1.8. By the up-flow fluidized bed reactor, the denitrification of wastewater containing 100 ppm nitrate-N, was successfully performed at a dilution rate of 2 d-1 with a trace of nitrate present in the effluent. Among these three ways of denitrification using cells of Pseudomonas fluorescence TIT16, the MBR system gave the most efficiency for the removal of nitrate-N in wastewater. Keywords: Pseudomonas fluorescence, denitrification, batch reaction, MBR system, fluidized bed, wastewater treatment Sheu Dey-Chyi 許垤棋 2001 學位論文 ; thesis 58 zh-TW |
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碩士 === 大同大學 === 生物工程研究所 === 89 === Abstract
In the process of organic wastewater treatment, the denitrification using cells of Pseudomonas fluorescence
TIT16 was investigated. Isopropanol served as electron donor and carbon source for denitrification. Three kinds of models, including a stirred tank batch reactor, a MBR system and an up-flow fluidized bed reactor, were provided for the studies of the optimum denitrification. For stirred tank batch reaction, the optimum pH was 7.5. After a 12-hour denitrification at pH 7.5, nitrate-N dropped from 140 to 10 ppm. Whereas nitrite was not detected and bacteria sustained life throughout the denitrification process. At pH 6.5, under a slightly acidic condition, the decrease of nitrate-N occurred slowly. The decrease of nitrate-N began at hour 8 and 50 ppm nitrate-N was left behind at hour 12. Meanwhile, the bacteria cells have decayed. At pH 8.5, under a slightly alkaline condition, nitrate-N held still and the bacteria died after a 24-hour denitrification was carried out. By MBR system at a bacteria dose of 0.54 g l-1 cell dry weight, the maximum dilution rate was 8 d-1 for the feeding of wastewater containing 80 ppm nitrate-N. The nitrate-N of the effluent was as low as 10 ppm and the bacteria cells kept alive. With the feeding of wastewater containing 80 ppm nitrate-N, when the dilution rate was shifted to 10 d-1, 40 ppm nitrate-N in the effluent was detected and the bacteria was dying according to the decrease of OD600 value from1.4 to 0.2. In the case of wastewater containing 60 ppm nitrate-N and at a dilution rate of 10 d-1, the effluent contained a nitrate-N below 10 ppm and the OD600 value held at 1.8. By the up-flow fluidized bed reactor, the denitrification of wastewater containing 100 ppm nitrate-N, was successfully performed at a dilution rate of 2 d-1 with a trace of nitrate present in the effluent. Among these three ways of denitrification using cells of Pseudomonas fluorescence TIT16, the MBR system gave the most efficiency for the removal of nitrate-N in wastewater.
Keywords: Pseudomonas fluorescence, denitrification, batch reaction, MBR system, fluidized bed, wastewater treatment
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author2 |
Sheu Dey-Chyi |
author_facet |
Sheu Dey-Chyi Hsieh Pei-Hua 謝佩華 |
author |
Hsieh Pei-Hua 謝佩華 |
spellingShingle |
Hsieh Pei-Hua 謝佩華 Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
author_sort |
Hsieh Pei-Hua |
title |
Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
title_short |
Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
title_full |
Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
title_fullStr |
Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
title_full_unstemmed |
Studies on Denitrification of Organic Wastewater by Pseudomonas fluorescence TIT16 |
title_sort |
studies on denitrification of organic wastewater by pseudomonas fluorescence tit16 |
publishDate |
2001 |
url |
http://ndltd.ncl.edu.tw/handle/64316608452642299812 |
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