| Summary: | 碩士 === 台北醫學院 === 保健營養學研究所 === 89 === Abstract
Glucosinolate derivatives, including ß-phenylethyl isothiocyanate (PEITC), indole-3-carbinol (I3C) and indolo[3,2-b]carbazole (ICZ), are thought to be the bioactive components in cruciferous vegetables. Pieces of evidence indicate that certain biological effect evoked by cruciferous vegetable derivatives is closely associated with nitric oxide (NO). In the present study, we investigated the glucosinolate derivatives PEITC, I3C and ICZ on the inhibition of the nitric oxide production in lipopolysaccharide (LPS) plus Interferon-γ(IFN-γ) stimulated RAW 264.7 cells. The results showed that PEITC (5x10-11 ~ 10-5 M) or I3C (10-10 ~ 10-4 M) could inhibit LPS plus IFN-γinduced nitric oxide production in RAW 264.7 cells after 24 h treatment (p < 0.05), and PEITC at high concentrations showed a dose-dependent response. Whereas ICZ showed no such dose effects. Western blot and RT-PCR analysis indicated that PEITC and I3C could inhibit LPS plus IFN-γinduced expression of inducible nitric oxide synthase （iNOS）protein and mRNA, respectively. Parallel to the NO production, PEITC had a significant dose-dependent inhibition on the iNOS protein and mRNA. In conclusion, cruciferous vegetable derivatives, PEITC and I3C could suppress LPS plus IFN-γinduced NO production by inhibiting iNOS gene transcription. However, ICZ did not have the significant inhibitory effect.
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