Immunohistochemical analysis of cytokine profile and androgen receptor of drug induced gingival overgrowth

• Main Authors: Huang Wan-Teng, 黃萬騰
• Other Authors: 呂炫kun
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/03515427984046024392

碩士 === 台北醫學院 === 口腔復健醫學研究所 === 89 === Abstract The purpose of this study is try to compare the androgen receptor+ cell (AR) （﹪）,Th1/Th2 cytokine profile（﹪）:IL-2, IFN-γ, IL-4,IL-10,IL-13 in the patients with healthy periodontium（PD<3mm, age range30-47）,inflammatory tissues of patients with adult periodontitis（P） with PD>5mm,（n=15,age range28-55）,surgically extracted tooth group（S） with PD>9mm,（n=10,age range24-79）and nifedipine induced ginigval overgrowth（NIGO）with probing depth(PD)>5mm,（n=5,age range51-63）.After full ethical approval of patients was achieved , the clinical periodontal parameters - pocket depth（PD）, bleeding on probing（BOP）, and plaque control record were measured around the selected diseasd periodontal area. Gingival samples were harvested during periodontal surgery and fixed in buffered formalin. Gingival biopsies were further processed for immunohistochemical stain with primary antibody of AR,IL-2,IL-4,IFN-γ,IL-10,IL-13 antibody（Santa Cruz biotechnology, Inc. C.A. U.S.A.）by using LSAB® method（Dako） subsequently . The expression of AR,IL-2,IL-4,IFN-γ,IL-10,IL-13 positive cells was counted using grid scan by semiquantitative method. The Kruskall—Wallis test , Mann-Whitney U-test were employed for the statistical analysis. The results revealed that AR, IL-2 ,IL-4,IFN-γ,IL-10,IL-13 was intensively expressed in the nuclei of basal epithelial cells, spinosal cells ,endothelial cell ,inflammatory cells ,and gingival fibroblasts .Strong expression of AR,IFN-γ,IL-2,IL-4 were also found in the NIGO group. Percentage （﹪）of AR labeled cells were significantly higher in the NIGO group of epithelial cells and gingival fibriblasts（58.6+2.3；80.2+10.7） than in the periodontitis group （56.3+3.3；52.5+11.8）and control（38.5+4.6；37.4+11.3）(P<0.05).In the surgically extracted tooth group ,very strong expression of Th2 （IL-4,Il-10,IL-13）type cytokine were found in the inflammatory cell than the Th1（IL-2,IFN-γ） type cytokine .The immuno expression of NIGO group has trend toward to the Th1 type cytokine（IL-2:p<0.01）expression .In the periodontitis group after periodontal phase I therapy was found strongly expression of IFN-γin the inflammatory cell. It seems that as at the quiesence stage of periodontitis , periodontium has the tendency to develope to the direction of tissue repair . Based on these findings , we postulated that AR and cytokine profile might play an important role in the progression of periodontitis and overgrowth of gingiva in NIGO. Key words: gingival overgrowth, androgen receptor, periodontitis, immunohistochemistry, cytokine profile。