Purification and Characterization of a-D-Galactosidase from Rice

• Main Authors: Peir - Ren Sun, 孫培仁
• Other Authors: Su-Fang Chien
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/87424920014437448554

碩士 === 淡江大學 === 化學學系 === 89 === Rice is a rich source of a-D-galactoside galactohydrolases （a-D-galactosidase EC 3.2.1.22）that are very widely distributed in nature(1) and may be important in the metabolism of various galactose-cotaining compounds in the animal and plant kingdoms. Therefore the purpose of this experiment is to purify the enzyme a-D-galactosidases from rice. The formⅠa-D-galactosidases was purified by extraction , gel filtration on Sephadex G-75、G-100 column, and ion exchange chromatography on DEAE-Sephadex A-50 、CM-Sephadex C-50 column. Five a-D-galactosidases from rice were identified by ion exchange chromatography.It was purified with a 104-fold increase in specific activity. The yield was 24% and the specific activity was 1.04 unit/mg protein. The molecular weight of the enzyme was estimated to be 25 kDa by Sephadex G100 column.