Regulation of Growth Performance and Body Composition by Expression of m-calpain Antisense RNA in Transgenic Mice

• Main Authors: SzuHuaChen, 陳思樺
• Other Authors: BorRungOu
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/33774795978863520068

碩士 === 東海大學 === 畜產學系 === 89 === The objective of this study was to investigate the effects of m-calpain antisense RNA on growth performance and body composition in transgenic mice. M-calpain cDNA fragment was inserted into pMAMneo vector, then the transgene was transferred into mouse embryo by microinjection. Transgenic mice were identified by polymerase chain reaction (PCR) and Southern blot. The body weights of transgenic male mice at 2.5 month old were higher than those of non-transgenic male mice at the same age. In addition, the body weights of transgenic female mice at 3 month old were higher than those of non-transgenic female mice at the same age. The concentrations of m-calpain in various tissues were determined by Western blot. To understand the possible mechanism, the body composition, water content, crude fat content and crude protein content were examined as well . There was no difference in water content and crude protein content between transgenic and non-transgenic mice. However, The crude fat content in transgenic mice was higher than those in non-transgenic mice. This result implied that the energy saved from reduction of protein degradation contributed to the accumulation of adipose tissue in transgenic mice.