Studies on the Chemical Constituents and Bioactivities of Vernonia Cinerea Less.

• Main Authors: Ang-Su,Yu, 余昂俗
• Other Authors: Shyh-Yuan Li
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/01914069295546761824

碩士 === 中國文化大學 === 應用化學研究所 === 89 === Abstract As a result of our continuing search for novel plant bioactive agents from plants , nine compounds including three steroids ： β-sitosterol (1)、sitgmasterol (3) and O-acetyl-sitgmasterol (9) , one triterpenes： Lupeol (2) , and one aromatic carboxylic acids： 4-octadecyloxy benzoic acid (4) , one aromatic esters： bis-(2- ethylhexyl)-phthalate (5)；and three Sesquiterpene lactones ： 8α-tigloyloxy-1—oxylmethyl- hirsutinolide (6)、2-methyl -but-2-enoic acid 11-hydroxy-6-hydroxymethyl -1,10-dimethyl-5 -oxo-4,14-dioxa- tricyclo [9.2.1.03,7] tetradeca- 2,6 -dien-8-yl ester (7) and 8α-tigloyloxyhirsutinolide-13-O-acetate (8) were isolated and characterized from Vernonia cinerea　 Less. (ASTERACEAE). Among them , compound 6 was isolated for the first time from natural source . The structural elucidation of these natural products were established by spectroscopic and chemical methods . Biological evaluation showed that compound 6 and compound 8 demonstrated cytotoxicity against human cervix epithelioid carcinoma（HeLa）, human colon adenocarcinoma（DLD-1）, human oral epidermoid carcinoma（KB）, human lung large cell carcinoma（NCI）and mouse hepatoma (Hepa) . Moreover compound 8 was demonstrated to exhibit the inhibition of DNA Topoisomerase Ⅰ .As to the bioassay of NO activity of the compound 8 is currently under investigation .