| Summary: | 碩士 === 中國文化大學 === 應用化學研究所 === 89 === Abstract
Asthmatic patients'' respiratory epithelium excrete nitric oxide (NO) due to airway inflammation. Thus, we use chemiluminesce method to measure the concentration of exhaled nitric oxide (ENO) that is a maker of airway inflammation.
In this thesis, there were two methods were performed for measurement of ENO. 1. ENO was performed via a on-line restricted exhaled breath. 2. Subjects exhaled air was collected into a Mylar bag, and then analyzed the ENO of the Mylar bag. We measured 45 normal subjects whose on-line ENO mean level was 12.562±1.032ppb and BAG NO mean level was 5.973±0.411ppb. We also measured 59 asthmatic patients who treated with steroids. ENO and pulmonary function test (FEV1) were measured for 8 weeks. Their baseline mean level of on-line ENO was 30.844±3.855ppb and BAG NO was 18.103±2.352ppb . Results show the asthmatic patients'' on-line ENO and BAG NO were higher than healthy controls and there was statistically significant change (p＜0.05). On-line ENO level was higher than BAG NO level. There was Statistically significant change (p＜0.05) and a correlation between them
(r＞0.5).
After steroids treated, asthmatic subjects'' the level of on-line ENO and BAG NO fell at wk2、wk3、wk4, increased at wk5、wk6 and fell at wk7、wk8 again. But there was no significant correlate between steroids and ENO and BAG NO of every week (p＞0.05).There was no statistically significant change between FEV1 and ENO, too ( P＞0.05).
Our conclusion is that asthmatic subjects'' ENO was higher than normal subjects. The level of on-line ENO was higher BAG NO and there was a correlation between them. After steroids treated, the level of ENO was unstable. There was no significant correlated between ENO and FEV1.
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