| Summary: | 碩士 === 中國文化大學 === 生活應用科學研究所 === 89 === The aim of this study was to evaluate the antioxidative substances, antioxidative functions and antioxidative capacity of nine herb plants, including Plantago asiatica Linn., Bidens pilosa Linn., Lycium chinense Mall., Ipomoea batatas Linn., Lilium formosanum Wall., Centella asiatica（L.）Urban., Mentha crispa Menth., Mentha arvensis L. var. piperascens Malinv., Mesona procumbens Hemsley.. The antioxidative substances include flavonoids, tannin, porphyrins, carotenoids, polyphenol. The antioxidative functions, such as reducing power, chelating Fe2+ ion, scavenging of DPPH radicals and superoxide anion were determined. The antioxidative capacity includes the inhibition percentage of conjugated diene formation in the linoleic acid emulsion system and the delay of lag phase of LDL. The correlation between antioxidative compositions, antioxidative functions and antioxidative capacity is analyzed. Meanwhile, the grey relation analysis and grey decision analysis are employed to analyze the interaction among the three.
In results, the porphyrins content was rich in Centella asiatica（L.）Urban. and Mentha crispa Menth. The carotenoids content was rich in Plantago asiatica Linn., Lilium formosanum Wall. and Centella asiatica（L.）Urban. The polyphenol content was rich in Plantago asiatica Linn., Bidens pilosa Linn., Ipomoea batatas Linn. and Mesona procumbens Hemsley.. In the antioxidative functions of aqueous extracts, Centella asiatica （L.） Urban. and Mesona procumbens Hemsley. had higher reducing power. Bidens pilosa Linn., Centella asiatica （L.）Urban. and Mesona procumbens Hemsley. were high in scavenging DPPH radicals activity. Bidens pilosa Linn. and Mesona procumbens Hemsley. were high in chelating Fe2+ activity. Lycium chinense Mall. and Mesona procumbens Hemsley. were high in scavenging superoxide anion. In the antioxidative functions of methanolic extracts, Plantago asiatica Linn., Centella asiatica（L.） Urban., and Mentha crispa Menth. had higher reducing power and scavenging DPPH radicals capacities. Lilium formosanum Wall. is the best in chelating Fe2+ and scavenging superoxide anion.
In the inhibition percentage of conjugated diene formation treated with various aqueous extracts of herb plants, Centella asiatica （L.） Urban. had the best inhibition percentage. The methanolic extracts of Plantago asiatica Linn., Bidens pilosa Linn. and Ipomoea batatas Linn. inhibited conjugated diene formation were higher than those of others. Centella asiatica （L.）Urban., then Mesona procumbens Hemsley., was better in the delay of lag phase of LDL treated with aqueous extracts while Plantago asiatica Linn. and Centella asiatica （L.）Urban. were better in methanolic extracts.
The content of tannin and polyphenol was positive correlated with the inhibition percentage of conjugated diene formation treated with various methanolic extracts. The porphyrins of aqueous erxtracts was negative correlated with the delay of lag phase of LDL while polyphenol showed positive correlation.
In the analysis of grey relational value and grey relational order between antioxidative functions and antioxidative substances, in aqueous extracts, polyphenol contributed to the reducing power and scavenging DPPH radicals and tannin contributed to chelating Fe2+ ion. Meanwhile, flavonoids contributed to scavenging superoxide anion. Among methanolic extracts, polyphenol contributed to reducing power and flavonoids contributed to scavenging DPPH radicals. Meanwhile, flavonoids and porphyrins contributed to chelating Fe2+ ion and flavonoids also contributed to scavenging superoxide anion.
In the grey relation analysis between antioxidative capacity and antioxidative substances, flavonoids contributed to antioxidative capacity of aqueous extracts in the linoleic acid emulsion system while flavonoids and tannin contributed to antioxidative capacity of methanolic extracts. In grey relation analysis between the delay of lag phase of LDL and antioxidative substances, both aqueous and methanolic extracts of polyphenol contributed to the antioxidative capacity.
In the grey relation analysis between antioxidative functions and antioxidative capacity, reducing power and scavenging superoxide anion capacity made contribution to antioxidative capacity for both aqueous and methanolic extracts in the linoleic acid emulsion system. In the LDL system, reducing power and scavenging DPPH radicals in aqueous extracts, and reducing power in methanolic extracts, made the contribution.
In the grey decision of the aqueous extracts, Bidens pilosa Linn. is the best, then Mesona procumbens Hemsley. and Centella asiatica（L.）Urban.. In the grey decision of the methanolic extracts, Bidens pilosa Linn. is the best, then Ipomoea batatas Linn. and Mentha crispa Menth.
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