Phylogenetic variations of microsatellite polymorphism on local porcine breeds in Taiwan

• Main Authors: Chia-Ching Wang, 王嘉慶
• Other Authors: Chun-Fan Chang, Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/39247741002295012015

碩士 === 中國文化大學 === 生物科技研究所 === 89 === The thesis study is to explore genetic relationship of porcine breeds by microsatellite DNA polymorphism. Genomic DNA from porcine breeds including Taiwan local strain (Lanyu), China strains (Meishan and Taoyuan), western strains (Berkshire, Landrace, Yorkshire, and Duroc), and as well hybrid strains (Spotty, Mitsai, and Lee-Sung) were employed for microsatellite DNA amplification, NTSYS, Winboot, and AMOVA analysis. The absence or presence of allelic microsatellite DNA bands are recorded in 0/1 binary format for calculating genetic distance among porcine strains. Three major clusters were revealed in both UPGMA and Winboot dendrogram. Cluster 1 (at similarity 0.37) consists of Lanyu, Spotty, Mitsai, and Lee-Sung porcine strains in the UPGMA dendrogram by DICE coefficient. Phylogenetic analysis also indicates a highly correlated cluster with the exclusion of Lee-Sung strain. Cluster 2 (at similarity 0.41) consists of Landrace, Yorkshire, and Duroc strains, whereas cluster 3 (at similarity 0.55) with Meishan and Taoyuan strains. Principal coordinate analyses (PCA) further distinguish porcine genetic clusters with respective zones: cluster 1 (-0.63 ~ -0.11, -0.22 ~ 0.17), cluster 2 (0.17 ~ 0.29, 0.06 ~ 0.60), and cluster 3 (0.17 ~ 0.52, -0.49 ~ -0.28). Moreover, AMOVA data concludes a 70.34% variance (p＜0.001) within porcine groups. Taken together, microsatellite DNA analysis efficiently reveals porcine development trend of Taiwan local strain, China strains, western strains, and hybrid strains, with which accords known historical records.