| Summary: | 碩士 === 國立臺灣大學 === 醫事技術學研究所 === 89 === ABSTRACT
Matrix metalloproteinases (MMPs) are believed to be important in the destruction and remodeling of the connective tissue and play important roles in tumor invasion and metastasis. Among MMPs, MMP-8 is able to decompose the major constituent of connective tissues, type I collagen, and MMP-9 is able to decompose gelatin. Chronic myeloid leukemia (CML) is a malignant clonal disorder of hematopoietic stem cells and demonstrates a biphasic or triphasic disease, which increases in myeloid cells not only in peripheral blood but also in the bone marrow. Since the role of MMPs in CML is not clear, this study is designated to find out the significance of MMP-8 and MMP-9 to CML patients.
In order to evaluate the possibility of differentiating CML from leukemoid reaction (LR), which also exhibits large proliferation of leukocytes, with MMP-8 and MMP-9, and to observe the change of MMP-8 and MMP-9 in the different stages of CML, we determined the concentrations of MMP-8 and MMP-9 in peripheral blood in CML patients and normal individuals by enzymatic immuno-analysis, and the MMP-8 and MMP-9 contents in neutrophils by flowcytometry. We also semi-quantified the mRNA in mononuclear cells (MNC) or granulcytes (PMN) by reverse-transcription polymerase chain reaction. We found that the plasma MMP-8 and MMP-9 concentrations and the MMP-8 and MMP-9 contents in neutrophils from the LR patients, which were not relevant to the absolute neutrophil counts, were parallel to the MMP-8 and MMP-9 mRNA expressed by PMN cells, and were higher than those in the CML patients or normal individuals. The CML patients with various stages showed no significant difference in MMP-8 and MMP-9 expression, except that one patient, whose leukemic status into AML, had a relatively low plasma MMP-9 concentration. However, the plasma MMP-8 concentrations from the newly diagnosed CML patients (CF) are parallel to the amount of MMP-8 mRNA existed in their mononuclear cell part. Additionally, in order to evaluate the possibility of using MMP-8 and MMP-9 to monitor the leukemic status of CML, we determined the plasma MMP-8 and MMP-9 levels in 4 patients prior to and during the conventional treatment. We found that after the treatment, the plasma MMP-8 and MMP-9 levels in the CML patients tended to decrease accompanied by the numbers and compositions of leukocytes approaching normal.
These results provided a preliminary information concerning the behavioral patterns of MMP-8 and MMP-9 in CML.
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