Detection of Herbicide-tolerant Genetically Modified Soybean from processed horticultural products

• Main Authors: Ching-Hsiang Hu, 胡慶翔
• Other Authors: Yuan-Tay Shyu
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/15546333912879100224

碩士 === 國立臺灣大學 === 園藝學研究所 === 89 === A method using PCR(Polymerase Chain Reaction) was designed for detection of herbicide-tolerant genetically modified soybean(Roundup Ready Soybean)，The presence of inserted DNA in soybeans could be detected with the three different primer-pairs specific for the inserted DNA of Roundup Ready soybean(RRS). The inserted DNA of RRS could be detected with primer-pairs 35S-f-EPSPS-r in soybeans containing 1% RRS and soybean milk / tofu made from soybeans containing 20% RRS. The commercial soybeans containing RRS more than 20%. Of 7 commercial soybean milk and 5 tofu samples , recombinant DNA was detected from 2 soybean milk samples and 2 tofu samples. 2 soybean milk samples were not detected. The other 3 soybean and 2 tofu samples should be non-GMO samples.