Detection of Herbicide-tolerant Genetically Modified Soybean from processed horticultural products
碩士 === 國立臺灣大學 === 園藝學研究所 === 89 === A method using PCR(Polymerase Chain Reaction) was designed for detection of herbicide-tolerant genetically modified soybean(Roundup Ready Soybean)，The presence of inserted DNA in soybeans could be detected with the three different primer-pairs specific for the ins...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
2001
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| Online Access: | http://ndltd.ncl.edu.tw/handle/15546333912879100224 |
| Summary: | 碩士 === 國立臺灣大學 === 園藝學研究所 === 89 === A method using PCR(Polymerase Chain Reaction) was designed for detection of herbicide-tolerant genetically modified soybean(Roundup Ready Soybean)，The presence of inserted DNA in soybeans could be detected with the three different primer-pairs specific for the inserted DNA of Roundup Ready soybean(RRS). The inserted DNA of RRS could be detected with primer-pairs 35S-f-EPSPS-r in soybeans containing 1% RRS and soybean milk / tofu made from soybeans containing 20% RRS. The commercial soybeans containing RRS more than 20%.
Of 7 commercial soybean milk and 5 tofu samples , recombinant DNA was detected from 2 soybean milk samples and 2 tofu samples. 2 soybean milk samples were not detected. The other 3 soybean and 2 tofu samples should be non-GMO samples.
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