Mass Propagation of Haemaria discolor (Ker-Gawler) Lindley and Haemaria discolor var. dawsoniana and the use of orchid mycorrhizal fungi.

• Main Authors: CHI-WEN CHANG, 張棋雯
• Other Authors: Doris C.N. Chang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/37943144199171164865

碩士 === 國立臺灣大學 === 園藝學研究所 === 89 === Haemaria discolor (Ker-Gawler) Lindley is also known as Ludisia discolor which was a terrestrial orchid and native habitat of Asia. It was well known for its attractive deep red leaves and leaf sheath. It could be used for ornamental and medical purposes. According to the flower odor, this plant was distinguished into Haemaria discolor (Ker-Gawler) Lindley (without flower odor) and Haemaria discolor var. dawsoniana (with flower odor). This thesis included four parts: 1. Literature review; 2. Asymbiotic and symbiotic seed germination of H. discolor var. dawsoniana; 3. Symbiotic culture and rhizomes propagation of H. discolor ;and 4. Effect of light, temperature, growth medium and fertilizer on the growth and development of H. discolor . Hand pollination after flowering for 0 to 24 days of Haemaria discolor var. dawsoniana was conducted. Seed germination rate (80.33%) as pollinated after flowering for 8 days was the highest. 1/4 MST medium (1/4 MS salt+3 g/L tryptone+30 g/L sucrose+7.8 g/L agar) resulted in better germination rate (84.73%) than 1,1/2 and 1/8 MST media. Hyponex No.3 agar medium (H3) was a simple sowing medium, in which the seed germination rate could reach 62.83%. Adding coconut (200 ml/L) and tomato (50 ml/L) juice in sowing medium could promote seed germination of H. discolor var. dawsoniana. The inoculation of orchid mycorrhizal fungi also could enhance seed germination , especially Rhizoctonia spp. coded R01 and R02 isolates in OMA medium. Seed of H. discolor inoculated with R01 or R02 could develope protocorm rapidly. Lliquid culture could enhance the rhizomes propagation of H. discolor. Adding Kinetin 5 mg/L and IBA 0.5 mg/L could form 6.2 times of lateral bud for 3 months. The inoculation of orchid mycorrhizal fungi (OMF) during transplanting could promote growth, and the presence of R02 isolate could reduce the use of 1/2 to 3/4 fertilizer of Johnson solution. Seedlings of H. discolor grew abundantly and has greener leaves which incresed ornamental quality under low light intensity (55.6μmolm-2s-1). Mass propagation and cultured conditions of H. discolor was intensively studied in this thesis. Due to the plants of H. discolor var. dawsoniana collected are limited, so only asymbiotic and symbiotic seed germination tests and optimal times of hand pollination were conducted. Tissue culture conditions and the use of orchid mycorrhizal fungi for this orchid needed further investigation.