| Summary: | 碩士 === 國立臺灣大學 === 昆蟲學研究所 === 89 === The phylogenetic relationships among Bemisia tabaci species complex (A and B biotype) and four rice grasshoppers (Oxya spp.) in Taiwan were analyzed by molecular markers (RAPD-PCR, rDNA, and mtDNA). The A and B biotype of B. tabaci could be discriminated by RAPD-PCR with four selected primers (OPA01, F12, 91167, and 91074). The result confirmed that both A and B biotype of this whitefly really exist in Taiwan. The specific primer set (Baf/Bar) was designed based on the nucleotide sequence of the amplicon, amplified by RAPD-PCR with primer 91074. This primer set, with the genomic DNA of B biotype of the whitefly, by PCR yielded an amplicon, about 500 bp, while nothing with that of A biotype whitefly was found. It revealed that this primer set can be specifically used to discriminate B biotype of the whitefly from A biotype.
Four rice grasshoppers (O. chinensis formosana, O. japonica japonica, O. podisma, and O. hyla intricata) were examined by RAPD-PCR with three selected primers (UBC431, OPA01 and OPA03). These four species could be distinguished based on the amplicon’s patterns. The phylogenetic relationships as analyzed: showed that O. chinensis formosana and O. japonica japonica are most closely related, followed by O. podisma and then O. hyla intricata. Comparisons and analyses of the nucleotide sequences of the ITS region of rDNA and the COI region of mtDNA of the four Oxya species showed that O. chinensis formosana and O. japonica japonica are closely related, followed by O. podisma and then O. hyla intricata. Therefore, O. japonica japonica is similar to O. chinensis formosana not only in morphology, but also in molecular markers. We concluded that O. chinensis formosana maybe a sibling species of O. japonica japonica.
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