| Summary: | 碩士 === 國立海洋大學 === 水產生物技術研究所 === 89 === High performance liquid chromatography (HPLC) is a widely used method for pigment analysis of marine phytoplankton. This method can separate and quantify most pigments, and the pigments as a tool to estimate the phytoplankton composition in the sea water. In this study, we refered to the publication of Joint Global Ocean Fluxes Study (JGOFS, 1994) and used the WRIGHT method to separate pigments in culture extracts from bacillariophyta, chlorophyta and cyanophta. Fucoxanthin, chlorophyll b and zeaxanthin were selected for the ratios calculation of chlorophyll a to fingerprint pigment for these three phytoplankton, and those were 1.39±0.03, 1.01±0.01 and 0.51±0.02, respectively.
A simple regression based on those ratios was used to estimated the contribution to total chlorophyll a in the East China Sea (ECS) during spring and autumn of 1998. In summer, these three phytoplankton generally provided over 90% of total chlorophyll a. While bacillariophyta dominated the near shore regime and cyanophta dominated the southeastern part of the ECS. In autumn, over 75% of total chlorophyll a were generated from those three phytoplankton in which bacillariophyta was the dominator in the most part of ECS, whereas cyanophyta appealed as dominator in the Kuroshio edge.
The counting results of synechococcus by microscopic method and chlorophyll a of cyanophyta by HPLC appealed positive correlation, expect the samples obtained in the regime of Taiwan Current Warm Water (TCWW). It revealed that pigment analysis by HPLC method can be a tool to estimate the phytoplanton community in future.
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