The Effect of chitosan hydrolysate digested by cellulase on intestinal microflora

• Main Author: 黃怡雯
• Other Authors: 蔡國珍
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/33329952026532813734

碩士 === 國立海洋大學 === 食品科學系 === 89 === The hydrolysate from celluase digestion on chitosan at 50℃ for 17 hr had the highest antibacterial activity. The antibacterial activity of these chitosan products against 1 strain of pathogenic Clostridium perfringens and 13 strains of probiotics, including 6 strains of Lactobacillus and 7 strains of Bifidobacterium were evaluated. In general, this chitooligosaccharide product had much strong antibacterial activity than the chitohexaose standars. C. perfringens was more susceptible to the chitosaccharide than the species of probiotics tested. The cell count was reduced by 8 log cycles with addition of 666 ppm of chitooligosaccharide; while only 1 log reduction was obtained for medium containing the chitohexose standard. The antibacterial activity of this chitosan hydrolysate against Lactobacillus was various with the species tested, with L. acidophilus and L. plantarum being most sensitive. No survival cell was detected at 666 ppm of chitooligosaccharide sample in contrast to 1 log reduction for chitohexose standard. The species of Bifidobacterium were very resistant to both the chitooligosaccharide and the chitohexose standard. The cell counts for B. longum and B. bifidum were similar in the control group and the group containing 1000 ppm of chitooligosaccharide. In another part of animal study hamster were divided into three groups for 4 weeks :1) 5% cellulose, 2) 5% fructo-oligosaccharides,3) chitosan hydrolysate. It was found that there were no significant differences among groups in daily feed intake, but chitosan hydrolysate will decrease the body weight. Fructo-oligosaccharides and chitosan hydrolysate groups had markly decreased liver TBARS levels. Plasma cholesterol concentration was markly decreased in hamsers fed with chitosan hydrolysate. A significant increase in the caecal count of Bifidobacterium was observed in the fructo-oligosaccharides and chitosan hydrolysate diets, and fructo-oligosaccharide decrease caecal pH significantly. The growth of three intestinal strains were evaluated in the basal medium with 0.5% carbon source. Fructo-oligosaccharides were most effective utilized by B. bifidum CCRC 14615 and B. longum CCRC 11847, but chitosan hydrolysate and chitohexose was found to be not utilized by these 2 strains. Presence of glucose, glucosamine, and fructo-oligosaccharide in the basal medium with GDCA and DCA will enhance the viability of B. bifidum CCRC 14615 and B. longum CCRC 11847. Presence of chitosan hydrolysate and chitohexose in the basal medium with GDCA will enhance the viability of B. bifidum CCRC 14615. The toxic effects of the bile acid on B. longum CCRC 11847 will be enhaced by the addition of chitosan hydrolysate.