| Summary: | 碩士 === 國立臺灣師範大學 === 生物研究所 === 89 === Abstract
In this study, we used electrophoretic technique to investigate the population genetic structure among 25 populations of Eumeces elegans in Penghu. Animals were collected from 23 islands of Penghu, 2 and 6 populations were collected from Marzu and Taiwan respectively for comparison. The total samples in this study are 310 individuals.
Twenty presumed gene loci resolved 52 alleles in 14 isozymes. The mean number of alleles per locus, the precentage of loci polymorphic, the mean observed and expected heterozygosity per locus of total population are A=2.6, P=50%, Ho=0.091 and He=0.191 respectively. The numbers of 25 populations in Penghu are A=2.3, P=45%, Ho=0.096 and He=0.177 respectively. The A, Ho and the number of genotypes were positively correlated with the area of islands (P<0.001, R=0.790; P<0.05, R=0.485; P<0.05, R=0.511). The A, P, Ho, He and the number of genotypes were positively correlated also with the island areas calculated depending on log10 (P<0.001, R=0.756; P<0.01, R=0.666; P<0.001, R=0.776; P<0.05, R=0.720; P<0.05, R=0.563).
The inbreeding coefficient (FIS) of Penghu and Taiwan populations is 0.3337 and 0.3826 respectively and it indicates that there is an obvious trend of intrapopulation inbreeding. The value of differentiation coefficient (FST) is 0.1867 and 0.1598 respectively and it indicates that the differentiation in Penghu is large. The mean value of gene flow (Nm) is 1.089 and 1.314 respectively and it indicates that the degree of gene flow is high.
The longest genetic distance is 0.279 and the smallest genetic identity is 0.757 among 33 populations in this study. The result from Neighbor-Joining analysis indicates that the E. elegans was differentiated into 3 groups. The 2 populations of Marzu were on the proximal branches. Six populations of Taiwan and populations of Penghu were on the other 2 groups. The differentiation influenced with MPI-1*、MPI-2*、GPI*. The genetic distance is positively correlated with geographic distance among all populations (P<0.001, R=0.604). This tendency was not observed in Penghu and Taiwan populations.
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