| Summary: | 碩士 === 國立中山大學 === 生物科學系研究所 === 89 === The IgE response increased in hosts infected with Angiostrongylus cantonensis. This study analyzes IgE responses appeared in different body fluids of mice infected with A. cantonensis.
BALB/c, CBA, C57BL/6 and C57BL/10 strain of mice were used in the experiment. BALB/c mice exhibited the highest susceptibility to A. cantonensis, CBA mice showed intermediate susceptibility, while C57BL/6 and C57BL/10 mice showed the lowest susceptibility. Analyses of specific antibodies against L5 antigens, and ES antigens in mice showed antibodies appeared in sera and in cerebrospinal fluids(CSF), especially IgE antibodies, were related to the worm rejection happened in mouse brain. However, antibodies appeared in ascites had no influence. The level of IgE increased in brain of susceptible BALB/c mice is much lower than that increased in other strain. Our data suggested that IgE response elicited in brain might be much more important than that in serum. Western blot analyses showed that among antigens extracted from L5 both IgE and IgG responses were positive in CSF. IgE antibodies against a 105 kDa protein of L5 antigen in C57BL/10 mice appeared in third week after infection, while in BALB/c mice appeared in fifth week. IgG antibodies against 105 kDa protein appeared in the identical weeks.
Our results indicated that 105 kDa protein was related to specific antibodies responses about protective function of mice infected with A. cantonensis. Further researches will be required to understand function and characterization of this protein.
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