Development of recognized materials for gas sensing by generation and screening combinatorial peptide libraries

• Main Authors: Jiing-Jong Wu, 吳景中
• Other Authors: Tzong-Zeng Wu
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/65437573512103581744

碩士 === 國立東華大學 === 生物技術研究所 === 89 === Combinatorial chemistry technology has played an important role in the field of pharmaceutical industry, especially in the identification and the optimization of lead compounds for drug design. Through high-throughput screening (HTS) process from synthesized large combinatorial chemistry library, the most optimal compound could be discovered. This method is considered as a novel approach since it can save time and resources in the process of new material development. Piezoelectric quartz crystal biosensor (PZ biosensor) is defined as a sensing tool that combines molecular recognition component of biomaterials with physical transducers. This study investigated the biomimic intimate molecule as recognized material for PZ biosensor by screening combinatorial peptide library. Various peptides coated PZ biosensors were used to detect volatile amines such as ammonia, monomethylamine (MMA), dimethylamine (DMA), trimethylamine (TMA). To determine pentapeptide sequence as recognized material for organic amines detection by PZ biosensor, 76 peptide combinatorial sub-libraries were synthesized and screened by iterative-selection and synthesis strategy. Furthermore, some volatile organic compounds (VOCs) including carboxylic acids, alcohols and aromatic compounds were also measured. From these detection, we can establish the peptide recognized materials vs. volatile organic compounds database. This study provides a new method to design peptide recognized materials for gas sensor. Noteworthily, this approach also has molecular diversity and experimental reasonableness advantages.