Determination of abuse drugs in urine by sequential injection analysis immunoaffinity mass spectrometry and organophosphorous pesticides residues by automatic renewable enzyme in sequential injection analysis

• Main Authors: chen chin-sheng, 陳晉昇
• Other Authors: 蘇宏基
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/65992754146523536384

碩士 === 國立東華大學 === 化學研究所 === 89 === An automatic sequential injection analysis (SIA) system coupling antibody reactor and the mass spectrometry was used of analyze D-methamphetamine, 3,4-Methylenedioxymethamphetamine (MDMA) and morphine in urine. This method was aimed at minimizing the steps of preatreatment and determining several analytes simultaneously. The detection limits are 50ppb for methamphetamine、MDMA and morphine, respectively. Linearity was observed in the concentration range 50-4000ppb for all analytes. The SIA system renewable based on inhibition acetylcholinesterase for organophosphorous determination was explored too. This system are feasible to renewable the enzyme reactor when enzyme was inhibited by pesticides during the analysis. Thus the time consuming regeneration step can be removed and maintain the activity of the enzyme every time in the reactor. The detection limits are 50ppb for paraoxon and 5ppb for carbofuran. Linearity was observed in the concentration range of 50-500ppb for paraoxon and 5-50ppb for carbofouran.