The study of the microbial community structure in the single-tank continuous inflow SBR

• Main Authors: Ping-Ting Huang, 黃璸珽
• Other Authors: Shu-Liang Liaw
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/56569801248915072376

碩士 === 國立中央大學 === 環境工程研究所 === 89 === In biological wastewater treatment processes, the structure of microbial community is one of the important factors which will affect operational performance of the processes. The structure, however, is very difficult to examine and monitor with traditional microbiological techniques, such as microscopy and cultivation. Recently, the microbial community examining techniques, based on PCR amplification of the 16S rDNA gene followed by denaturing gradient gel electrophoresis (DGGE) of the amplicons, has become a useful technique for study of the microbial community structure. Therefore, the purpose of this study is: (1) to find out the suitability of applying the PCR-DGGE technique to examine the microbial community structure of a single-tank continuous inflow SBR; (2) to find out the relationship between the microbial community structure and the removal efficiency; and (3) to find out the relationship between the microbial community structure and the pattern of ORP profile. The results have shown that the rapid shift of populations was observed in the period of the start-up stage, and succession toward a more stable community structure along with cultivation time. As a relatively stable assemblage of populations is achieved, the removal efficiency and the pattern of ORP profile are almost constant. In conclusion, the PCR-DGGE technique is a feasible technology for study of the dynamic variability of microbial community structure in the continuous-flow SBR system.