Stimulation of mitotic exit of arsenite-arrested metaphase cells by staurosporine

• Main Authors: Tang-Hui-Chen, 唐惠真
• Other Authors: Lee, Te-Chang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/50707414288819530133

碩士 === 國立中央大學 === 生命科學研究所 === 89 === Arsenical compounds are widely distributed in the water, soil and air. Epidemiological evidences have revealed that arsenic exposure is associated with increased incidences of a variety of diseases, such as Blackfoot Disease, and skin, liver, bladder and lung cancers. Previous studies in our laboratory have shown that treatment of HeLa S3 cells with 5 μM arsenite leads to about 35% of the cells arrested in the mitotic metaphase. Treatment of arsenite-arrested mitotic metaphase cells with staurosporine (protein kinase inhibitor) enforces them exiting from mitosis. In this study, we try to observe changes of ultrastructure and investigate protein modifications by staurosporine on arsenite-arrested mitotic cells. Our results show that treatment of arsenite-arrested mitotic cells with staurosporine leads to dissolution of chromosome and elongated and condensed spindle, reformation of nuclear envelope and generation of multiple nuclei. In addition, proteins modified by staurosporine treatment on arsenite-arrested mitotic cells are also observed by two-dimentional gel electrophoretic analysis. However, these proteins should be further identified and characterized by MALDI-TOF or 2D-Western techniques.