| Summary: | 碩士 === 國立交通大學 === 應用化學系 === 89 === Capillary electrophoresis and related techniques on microchips have made great strides in last decade. Typically, these chips were fabricated in glass or quartz because of their well-defined surfaces, good electroosmotic properties, and excellent optical properties for ultra sensitive detection. The analytes were commonly detected by laser-induced fluorescence (LIF). Due to the multi-fabrication process and the expensive detection system, the microelectrophoresis chip system is costly.
In this study, we used plastic chips combined chemiluminescence (CL) detection method to explore the application of microchip electrophoresis. The channels were fabricated on poly(methylmethacrylate) (PMMA) by wire imprinting method. The channel was constructed by heating the plastic at 102oC for 8 min. After forming the channel layout, the channel was sealed with another PMMA by heating the plastic at 104oC for 8 min. The luminol-H2O2-HRP system was employed for testing CL system. Several factors such as electric field strength, mixing mold of reagents, and detection point that affected the CL intensity were examined. At the optimized condition, the H2O2 linear range was 0.1% ~ 0.01% with theoretical detection limit at 0.001%.
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