The Chilling Effect on Rice Seedling--A Study from Protein Viewpoint
碩士 === 國立成功大學 === 化學系 === 89 === This study is to use proteomic method to investigate the chilling effect on dark-germinating rice seedling. Different length of coleoptiles (1, 2, 3 and 4 cm) obtained from normal seedling at room temperature were used to grow in 1 : 3 ratio of original g...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2001
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/11766058661039588894 |
| Summary: | 碩士 === 國立成功大學 === 化學系 === 89 === This study is to use proteomic method to investigate the chilling effect on dark-germinating rice seedling. Different length of coleoptiles (1, 2, 3 and 4 cm) obtained from normal seedling at room temperature were used to grow in 1 : 3 ratio of original growth water to fresh de-ionized water at 4 oC for various time intervals. Coleoptile elongation was observed, but with a slow rate compared with those grown at room temperature. The total protein extracts of coleoptiles for different length grown at different stage were examined to investigate the differences compared to those grown at room temperature. The extracted proteins was first treated with sodium sulfate precipitation method by using the concentration of sodium sulfate up to 95%. The 95% sodium sulfate co-precipitated proteins was then subjected to 2-D gel electrophoretic analysis. It was found an addition protein spot was observed for coleoptile grown at 4 oC. And the quantity of this protein is larger in 4 cm coleoptile than in shorter coleoptile and increases with the increase of grown time. It was found it become very obvious after 6 days of growth. The average survival rate under chilling condition was more than 90% after 6 days growth. Further identification of this protein spot with mass spectroscopic technique and library search found it is a new protein with molecular weight of about 16 kDa. These results indicate that chilling causes the physiological change of rice seedling in terms of proteomic view.
|
|---|