| Summary: | 碩士 === 國立中興大學 === 獸醫學系 === 89 === Infections of Muscovy duck parvovirus (MDPV) and goose parvovirus (GPV) are important diseases of waterfowls in Taiwan. These diseases not only lead to a high mortality in ducklings and goslings, but also cause growth retardation of adult birds and economic losses. Because no effective vaccination and rapid diagnosis method is available, these diseases impose a potential risk on waterfowls in Taiwan. The goal of this study is to understand the distribution of GPV and MDPV in infected birds, and to understand the antibody titers of GPV and MDPV in geese and ducks in Taiwan. We first collected and propagated GPV and MDPV isolated from recent years in Taiwan. We then infected mule ducks by GPV or MDPV by intranasal inoculation of 0.2 ml allantoic fluids containing GPV or MDPV. We examined the presence of virus in the infected birds by PCR. We found that viruses were present in the thymus, sinus nasalis, esophagus, and liver in the second week after infection. We also used E. coli expression system (pET32 system) to express the antigenic NS1 protein of GPV and MDPV. We found that ELISA plates coated with 40 ng of recombinant NS1 per well gave the best result for the detection of antibody against GPV and MDPV. By this ELISA, we examined the antibody titers against GPV and MDPV in sera collected from different areas of Taiwan. We found that the titer is low in birds from isolated islands (Kingman and Machu), probably due to the geographical separation of these islands from Taiwan. In comparison, the titer is relatively high in southern counties of Taiwan (Jaiyi, Tainan, Kaoshun, and Pington), probably because the conduction of traditional vaccination program has been disregarded in recent years. We conclude that the recombinant NS1 ELISA established in this study might save as an important tool for the diagnosis of GPV and MDPV infections in Taiwan.
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