Genetic variation of Salix warburgii and S. kusanoi based on ISSR markers

• Main Authors: Cheng-Shien Tsai, 蔡承憲
• Other Authors: Ju-Ying Hsiao
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/23508360082111992170

碩士 === 國立中興大學 === 植物學系 === 89 === Salix warburgii and Salix kusanoi (Salicaceae) are two hydrophilic species of the genus Salix in Taiwan. They are generally found in lake and river bank at the altitude below 1500 meters. S. kusanoi is a rare and en-dangerous species while S. warburgii has a wider distribution. In the present study, 144 individual samples from 12 populations of S. warburgii and 62 individuals from three populations of S. kusanoi were studied using ISSR markers. One-hundred and twenty-four ISSR primers were screened and primers that produced reproducible polymorphic bands were used. Eighteen primers were used for S. warburgii and 113 polymorphic bands were recorded. Fifty polymorphic bands were recorded in S. kusanoi using 15 primers. By employing POPGENE software, the gene diversity coefficient (Gst) of S. warburgii and S. kusanoi were 0.182 and 0.494. respectively. The analysis of molecular variance (AMOVA) revealed that the variance components among regions (north, central, south and east regions), among populations within regions, and among individuals within populations were 9.99% (p<0.001), 23.23% (p<0.001) and 66.78% (p<0.001) of the total variance, respectively in S. warburgii. In S. kusanoi, the variance components among populations and among individuals within populations were 59.70% (p<0.001) and 40.30% (p,0.001) of the total variance, respectively. The results of UPGMA cluster analysis and principal coordinate analysis indicated that, within S. warburgii, Tsusang and Tsuoyin populations were the two more isolated populations that may resulted from interspecies hybridization. S. kusano had greater diversity among populations which may resulted from longtime geographic isolation and limited gene flow among populations.