Mass rearing propagation of Amblyseius womersleyi Schica (Acari: Phytoseiidae) in a closed system

碩士 === 國立中興大學 === 昆蟲學系 === 89 === Abstract Amblyseius womersleyi (Schicha) was reared in a closed system in a vial ( 5.6 cm × 3.7 cm diam.) at 26 ± 1℃, 13L:11D and 50%, 60%, 70%, 80% and 90% RH. The highest egg hatching rate (100%) of A. womersleyi was found at 80% RH, which wa...

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Bibliographic Details
Main Authors: Li, Chia Hsing, 黎家興
Other Authors: Advisor: Chain-Ing Shih
Format: Others
Language:zh-TW
Published: 2001
Online Access:http://ndltd.ncl.edu.tw/handle/21326937320682893208
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Summary:碩士 === 國立中興大學 === 昆蟲學系 === 89 === Abstract Amblyseius womersleyi (Schicha) was reared in a closed system in a vial ( 5.6 cm × 3.7 cm diam.) at 26 ± 1℃, 13L:11D and 50%, 60%, 70%, 80% and 90% RH. The highest egg hatching rate (100%) of A. womersleyi was found at 80% RH, which was not significantly different from those tested at 70%, and 90% RH, but was higher than those at 50% and 60%. The optimal relative humidity to preserve the predaceous mites in a vial without prey and water was 90% RH. The days of 50% mortality reached for larvae, protonymphs, adult males and adult females were 2.6, 2.6, 4.4 and 3.7 days, respectively. With sufficient food supply, a high percentage of eggs, larvae, protonymphs and deutonymphs developed were found at 70 - 90 % RH. Relative humidity significantly influences the daily prey consumption rate of adult females of A. womersleyi, but not the daily fecundity. The maximal number of predators (1296 ± 161.7 mites/vial) were reared and reached at day 21, under a closed propagation conditions with daily food supply of 0.01g and 0.02g of eggs and larvae of Tetranychus urticae for 1-14 days and 15-21 days, respectively. The predator densities reached highest ( 1296±161.7 mites/vial ) at day 21. The advantages and superiorities of using closed rearing unit of a vial rather than open artificial stages were compared and discussed. 2. 往昔研究 3 2.1. 捕植蟎、葉蟎及二者間之相互作用 3 2.2. 二點葉蟎與溫氏捕植蟎之重要性 7 2.2.1. 二點葉蟎之重要性 7 2.2.2. 溫氏捕植蟎之重要性 8 2.3. 影響捕植蟎族群增殖力與捕食力之因素 9 2.3.1. 非生物因素 9 2.3.2. 生物因素 13 2.4. 大量飼育捕植蟎 17 3. 材料與方法 24 3.1. 材料 24 3.1.1. 食餌準備 24 3.1.2. 飼育載台及捕植蟎定齡 24 3.1.3. 育蟎瓶、單隻飼育管與給餌裝置 25 3.1.4. 溼度控制盒 26 3.2. 相對溼度對溫氏捕植蟎各齡期之影響 27 3.2.1. 卵孵化率 27 3.2.2. 各齡期蟎體成長百分率 27 3.2.3. 不給餌處理下各齡期蟎體之存活狀態 27 3.2.4. 雌成蟎之捕食量及產卵量 28 3.2.5. 相對溼度及給餌量對育蟎瓶內溫氏捕植蟎族群密度增殖之影響 28 4. 結果與討論 29 4.1. 相對溼度對溫氏捕植蟎各齡期之影響 29 4.1.1. 卵孵化率 29 4.1.2. 齡期發育成功百分率 29 4.1.3. 不給餌處理下各齡期存活狀態 31 4.1.4. 發育期所需時間 32 4.1.5. 雌成蟎每日捕食量及產卵量 33 4.2. 相對溼度對溫氏捕植蟎族群之影響 34 4.3. 不同給餌量對育蟎瓶內捕植蟎族群增長之影響 35 5. 結論 39 6. 參考文獻 ………………………………………………………………………39 中文摘要…………………………………………………………………….……….55 Abstract…………………………………………………………………………..…...56