Characteristic of Induced Phosphate-solubilizing Activities and Exudates of Bacteria

• Main Authors: Hsiao-Keng Tsai, 蔡效耕
• Other Authors: Chiu-Chung Young
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/83631179960190763305

碩士 === 國立中興大學 === 土壤環境科學系 === 89 === The non-phosphate-solubilizing bacteria may be induced by metabolites of ether phosphate-solubilizing bacteria. We try to investigate the characteristic of induced phosphate-solubilizing (IPS) activities and exudates of bacteria. The Pseudomonas cepacia Al74 and Escherichia coli JM109 were applied as standard IPS system for measuring the diameter between clear zone and colony. Besides, we analysised of organic acids and water-soluble phosphorus in tricalcium phosphate medium during 180 hr of 5 strain including phosphate- solubilizing bacteria (PSB) strain (Al74, bc29, and bc30), and Rhizobium sp. (A1, and A9) by HPLC and spectrometer after observation the phenomenon of 15 PSB and 24 Rhizobium strain on the tricalcium phosphate solid medium. In addition to activities and exudates study, glucose dehydrogenase (GDH)-related primers were used to study the genotype of 15 PSB strain. The result of IPS on the solid medium is related to bacteria species, and Rhizobium both having having IPS activity and phosphate-solubilizing ability. In standard IPS system, the ratio, which was used in quantilification of the effects of IPS, of phosphate-solubilizing ring to circle was related to IPS distance. In liquid system, co-incubation with E. coli JM109 were reduced the amount of water-soluble phosphorus at the initial stage, and increased later. Except polysaccharid-richness Rhizobium sp. A1, the secretions of Al74, bc29, bc30, and Rhizobium sp. A1 all have secreted 2-keto-D-gluconic acid, which was cope with phosphate-solubilizing hypothesis. The pattern of most PSB PCR products of dehydrogenase-related primers was variety. The primer PqC and GDH used in prediction IPS have correlation value over 0.73, and correlation between PqA and phosphate-solubilizing clear zone is 0.74. Therefore the pyrroloquinoline quinone, cofactor of GDH is one factor in the IPS system.