Study of Hyperpigmentation Mechanism in Basal Cell Carcinoma

• Main Authors: Chiu-Min Cheng, 鄭秋敏
• Other Authors: Hsin-Su Yu
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/55176300697951160051

碩士 === 高雄醫學大學 === 醫學研究所 === 89 === Basal cell carcinomas(BCCs) are one of the most common skin malignant diseases in human beings, BCC cells show a morphologic resemblance to the relatively undifferentiated cells of basal layer of the epidermis .It is only locally invasive and rarely metastasizes. Basal cell carcinoma*s incidence has risen theses last decades because of the increase in sun exposure habits. Most of BCCs in orientals are pigmented. A basal cell carcinoma cell line (BCC-1/KMC) has been successfully established from a pigmented patient. We compared normal keratinocytes with BCC-1/KMC to investigative the differences of BCC-1/KMC from normal keratinocytes. We collected the supernatants of BCC-1/KMC and keratinocytes irradiated by UVB, then added them to culture melanocytes. The results indicated that the supernatant of BCC-1/KMC irradiated by UVB increased viability, melanogenesis and migration of melanocytes. In addition, the cultured BCC cells spontaneously secreted more basic fibroblast growth factor (bFGF) and Endothelin-1(ET-1) than keratinocytes and UVB stimulated more increases in bFGF and ET-1 release from BCC-1/KMC than keratinocytes. bFGF and ET-1 are both mitogens for melanocytes, and ET-1 is a melanogen for melanocytes. Our results suggested that the hyperpigmentation of BCC may increase through the stimulation of melanocyte melanogenesis and migration by the action of bFGF and ET-1.