| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 89 === Euphorbia thymifolia Linn. (Euphorbiaceae) is a medicinal herb used as a diuretic and a laxative in Taiwan. In literature record, this crude drug has detumescence, anti-diarrhea, anti-malaria, anti-rash, anti-dysentery, anti-carbuncle, detoxify and anti-hemorrhoids. Recent studies showed that the causes of many diseases are related with excess free radicals in body, while viruses infection will accomplish with the production of free radicals. To evaluate the antioxidant and anti-HSV activities of E. thymifolia, the following studies will be evaluated:
1.FeCl2-Ascorbic Acid Test.
2.Cytochrome C Test.
3.Xanthine Oxidase Inhibition Test.
4.Electron Spin Resonance（ESR）
5.Antiviral Assay and Cytotoxicity Assay.
The results showed that Euphorbia thymifolia Linn. exhibited an antioxidant and anti-HSV-2 activity. The range of IC50 for fractions during FeCl2-Ascorbic acid test, Xanthine Oxidase Inhibition Test and Cytochrome C Test were 2.81~7.63, 0.03~32.13 and 0.013~0.410 mg/ml, while for pure compounds were 3.69~5.72, 0.36~2.33 and 0.023~3.650 mM, respectively. ESR test results showed that water fraction and pure compounds of E. thymifolia Linn. possessed SOD-like activity. However, the hydroxyl radical scavenger activities of E. thymifolia was weak. Besides antioxidant activities, EtOAc fraction and 3-O-galloyl-4, 6-(s)-HHDP-D-glucose also exhibited anti-HSV-2 activity. None of the fractions and pure compounds of E. thymifolia possessed anti-HSV-1 activity.
The results show that E. thymifolia has exhibited anti-lipid peroxidation, superoxide anion radical scavenger, superoxide anion radical formation inhibition, SOD-like and anti-HSV-2 activities.
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